A commonly used chromatographic method is the Liquid Chromatography. HPLC stands for High Performance Liquid Chromatography.
HPLC consist of elution, pump systems, injection systems, a column, a detector and a computer. The elution usually consists of methanol and water. The pump system composes the elution. If we inject the required liquid into the injection system, the elusion will be pumped to the injection system and from there to the column. In the column, the molecules cannot penetrate easily, resulting in the need for high pressure. The small and easily soluble molecules will be the first to leave the column while larger molecules and poorly soluble molecules detected by the detector are the last to leave the column. If the detector detects a substance, it sends the information of the computer. The computer shows a graph with the time for a specific substance set out on the horizontal axis. The time in which a specific substance leaves the column is always the same – under the same circumstances – and it is called the retention time. If we know the retention time of a particular substance under these circumstances, then we can immediately see if the mix contains that substance. However, we don’t know yet in which concentration and therefore the peak surface area has to be calculated and a calibration curve must be created.
High-performance liquid chromatography (HPLC) is a separation method; it is liquid chromatography in which the elution (the liquid solvent) is pumped under high pressure through a tightly packed column.
The advantages of HPLC
The measurements can be carried out rapidly and many analyses can be done consecutively. This means that the method is hardly labour intensive. It is very accurate and even the smallest quantities can be detected and determined.
QUANTOS is a measuring device that was developed by Q-FOOD GmbH in Freiburg (Germany).
By means of the QUANTOS, a mid-infrared FTIR spectrometer, we can measure several parameters in energy drinks, soft drinks and beers within several minutes with an accuracy of 1 ppm (parts per million).
We use the Quantos to determine within minutes the various sugars, sorbic acid, benzoic acid, alcohol, taurine, caffeine, citric acid, phosphoric acid and acesulfame-K. This is a great advantage for our clients. Normally they have to wait days and sometimes weeks until results come in from external labs.
The methanol content in the alcohol mixes and the sugar and alcohol content in Radler beers can also be determined very easily.
By deploying QUANTOS, DIS can respond better to any changes in legislation governing taurine and methanol, the evidence of sweeteners, the absence of sugars and the burden of proof for Halal.